Here, a fluorophore, BODIPY 493/503, displayed altered solid-state emission characteristics upon being hosted within a crystalline molecular flask. The emission was collected through a 545/65 band pass filter. Shop a large selection of products and learn more about Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) Description: With its nonpolar structure and long wavelength absorption and fluorescence BODIPY 493 503 can be used as a stain for netural lipds and as a tracer for oil and other nonpolar lipids. … Mengliang Zhu, Peipei Xing, Yabin Zhou, Lei Gong, Jinghui Zhang, Dongdong Qi, Yongzhong Bian, Hongwu Du, Jianzhuang Jiang, Lysosome-targeting ratiometric fluorescent pH probes based on long-wavelength BODIPY, Journal of Materials Chemistry B, 10.1039/C8TB00883C, 6, … Due to high lipophilicity, some BODIPY derivatives tend to adhere to and penetrate the cell membrane and accumulate in subcellular membranes ( 11 ). BODIPY 493/503 can be use to create electronicaly neutral green-fluorescent probes. BODIPY Version: 1 Modified from: Edited by: Dr. Rutkowsky, UC Davis Summary Reagents and Materials Protocol Reagent Preparation Reagent 1 Reagent 2 Reagent 3 Summary: BODIPY® 493/503 can be used as a stain for neutral lipids and as a tracer for oil and other nonpolar lipids. Ex/Em = 493/503. Figure Legend Snippet: Bodipy-493/503 versus Oil Red O. How does it work? COVID-19 is an emerging, rapidly evolving situation. The dye is convenient for double fluorescence labeling, but we noticed that it emits red fluorescence under … quenching. BODIPY (493/503) contains a nonpolar structure that, upon binding to neutral lipid, emits a green fluorescence signal with a narrow wavelength range, making it an ideal approach for using in multi-labeling (or dye) experiments . Figure 7. Furthermore, because DAPI required a longer time to stain M. oryzae cells compared with the other dyes (BODIPY 493/503, Calcofluor white and Nile red), especially in staining for M. oryzae conidia, we had to increase the staining time or the concentration of DAPI solutions to obtain good images. How big is it? BODIPY 493/503 methyl bromide can be used to label thiols with the green-fluorescent label. What and where is thefluorescent moiety? We assessed both fixed (4% paraformalde- Here, a fluorophore, BODIPY 493/503, displayed altered solid-state emission characteristics upon being hosted within a crystalline molecular flask. BODIPY ® 493/503 - (O-0034) --> Skip to the beginning of the images gallery. . Photochemical properties & single-to-background ratio (SBR) for the BAA library. BODIPY 493/503. With its long-wavelength absorption and fluorescence, BODIPY 493/503 can be used as a stain for netural lipds and as a tracer for oil and other nonpolar lipids. Exportable as image or link. BODIPY 493/503 or Nile Red (neutral lipid) for 30 minutes. pH-insensitive fluorescence; Narrow spectral bandwidth; Higher 488 nm absorptivity than the BODIPY FL fluorophore Specifically, a bathochromic shift of 939 cm-1 was observed (λ(max): 633 → 673 nm), with a concomitant reduction in emission intensity, and emission dependency on excitation wavelength. Is it metabolized? Emission Wavelength Maximum: 506 nm : Absorption Wavelength Maximum (Abs. The excitation wavelength was 485-nm with a 1mm slit size. Therefore, the staining characteristics of BODIPY 493/503 are better than those of Nile red for PHB analysis. Customers also purchased with this product. Ex/Em = approx 558/568; emits into red region. Description. BODIPY 493/503 is a BODIPY dye in which the 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene skeleton is substituted at positions 1, 3, 5, 7, and 8 by methyl groups. Ask an Expert. Additionally, because the emission spectrum of BODIPY 493/503 is at a lower wavelength than that of Nile red, the use of this dye allows additional flexibility … excitation wavelength was 470-nm. Consecutive cryosections from human VL muscle were stained with ORO or Bodipy-493/503. 1,2 BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. How long does is stay in the “lipid” of the cell? The (A) maximum absorbance vs. maximum emission wavelength, (B) quantum yield (Φ fl) vs. maximum emission wavelength, (C) full-width-at-half maximum (FWHM) vs. maximum emission wavelength, and (D) SBR vs. maximum emission wavelength were calculated for the BAA library and compared to BODIPY … BDP 493/503 is a staining dye for lipids that is useful for microscopy, staining and tracing of hydrophobic media. BODIPY (D3922, Molecular Probes, Carlsbad, Calif, USA) (excitation wavelength 480 nm, emission maximum 515 nm), was diluted in PBS or DMSO at a concentration of 1 mg/mL and applied to cross sections or FDB fibers, respectively, for 30 mins. Download in ChemDraw format. BODIPY 558/568 C 12: Stock aliquots in DMSO (-30°C freezer) at 2.12 mM. (2010) stained lipid droplets in mammalian cells using BODIPY 493/503, Cooper et al. BODIPY (493/503) Staining and Visualization. Cell Analysis, Cellular Imaging, Immunofluorescence (IF), Immunofluorescence Staining & Detection. The lipid droplet (LD) has become a focus of intense research. Does it stain all membranes? BODIPY derivatives have been used in the development of fluorescent sensors and indicators for pH, metal ions, reactive oxygen species (ROS), nitric oxide (NO), redox potential, etc. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. It is used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids. Ohsaki et al. BODIPY 500/510 spectrum - BODIPY 500/510 is a fluor... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells. 2.4. Lambda stacks of the fluorescence emitted by Bodipy and ORO stained muscle sections were acquired with a LSM780, with a resolution of 9 nm (A). BDP TR methyl ester, 5 mM in DMSO BDP TR methyl ester is a counterstain for GFP. Results: BODIPY 493/503 staining had less background staining, higher sensitivity and specificity to PHB, and higher saturation values than did Nile red staining. This product(s) resides on a … Item BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) Company Thermo Fisher Scientific; Catalog Number D3922; This product is no longer available on Biocompare. How specific is it. Max) 500 nm : Oligo Modification Type: Fluorescent Dye (Green/Yellow) FAQs . Cells were then imaged using a Cytation 5 and images analyzed with Gen5™ Microplate Reader and Imager Software. BODIPY (D3922, Molecular Probes, Carlsbad, Calif, USA) (excita-tion wavelength 480nm, emission maximum 515nm), was diluted in PBS or DMSO at a concentration of 1mg/mL and applied to cross sections or FDB fibers, respectively, for 30mins. Working solutions range around 10-80 uM but you should experiment to determine the best staining for your sample. BODIPY 493/503 - Ontology Report - Chinchilla Research Resource Database × Welcome {{ username}} Message Center {{ messageCount }} Messages. Materials and Methods Cell Culture HepG2 cells were cultured in Advanced DMEM sup-plemented with 10% fetal bovine serum and penicil-lin-streptomycin at 37 °C in 5% CO 2 Lipid Rafts? 2.4. BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells. A 5 mM solution in DMSO. Bodipy | C9H7BF2N2 | CID 25058173 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more. BODIPY FL C5-ceramide complexed to BSA: Reconstitute 5 mg unit in 150 µM sterile distilled water. BODIPY (493/503) Staining and Visualization. Staining with BODIPY 493/503 (D-3922) has been shown by flow cytometry to be more specific for cellular lipid droplets than staining with nile red.19 Mark Cooper at University of Washington has found that BODIPY 505/515 (D-3921) rap-idly permeates cell membranes of live zebrafish embryos, selec-tively staining cytoplasmic yolk platelets. Does it extend beyond the “plane” of the membrane? The data is best fitted with a single exponential decay time of 2.53 ns (x² = 1.04). BODIPY 493/503, however, has some aberrant fluorescent properties that must be considered prior to multicolor fluorescent analysis . Frequency responses (phase and modulation) of Alexa 546 in water acquired on K2 using a Xenon lamp. Fluorescence labeling is indispensable for the cell biological analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used extensively for LD labeling. Search results for bodipy-C11 fluorescence at Sigma-Aldrich BODIPY 493 503 4 4 Difluoro 1 3 5 7 8 Pentamethyl 4 Bora 3a 4a Diaza s Indacene. BODIPY 493/503 is a FITC-range dye with greater LD specificity and a smaller fluorescent spectral footprint than Nile Red (9,23). Bio-Synthesis offer BODIPY 493/503 fluorescent dye oligonucleotide labeling services. BODIPY 493/503 have mostly been used, as they fluoresce in a hydrophobic environment such as in the core of an LD.8,9 However, one of their drawbacks is the fluorescence back-ground resulting from nonspecific binding to lipophilic sites other than an LD in a … [1],[2] BODIPY 493/503 is compatible with epifluorescent, confocal, … Used to label thiols with the green-fluorescent label DMSO ( -30°C freezer ) at 2.12 mM Analysis. Gen5™ Microplate Reader and Imager Software emission was collected through a 545/65 band pass.! K2 using a Xenon lamp lipid ” of the cell in water acquired on K2 using a Cytation 5 images! 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