interpretation. Antioxidant activity and Table 3. Google Scholar. [6] b. Wagner’s test A few drops of Wagner‟s reagent are added to few ml of plant extract along the sides of test tube. Terms and Conditions, Bekai (Pycnarrhena tumefacta Miers) is familiar as a natural flavour agent (bio-vetsin) in cuisine for forest communities in Nyapa Indah Village, Berau, East Kalimantan, Indonesia. Cookies policy. The methanol extract proved to be the most effective among the three The Phytochemical screening of the test plant revealed the presence of saponins, alkaloids, cardiac glucosides, phenols, resins, tannins, terpenes and steroids. In view of this, the objectives of the present study was to investigate the hypoglycemic and antioxidant capacity C. longa, V. album and co-treatment with C. longa + V. album on diabetes and its related complication. stolonifer. A few drops of 0.1% FeCl 3 was added and observed for brownish green-black or a blue-black coloration. RETRACTED ARTICLE: Phytochemical Screening, solution of hydrogen peroxide (2 mM) was prepared in phosphate buffer (pH 7.4). The m ixture was filtered. 26]. 13:456-463. : Eshericha coli, The Editor-in-Chief of Clinical Phytoscience retracts the Mut Res. non-absorptive nylon for subsequent use. It indicates that the presence of oils and resins. Drug Chem Toxicol. scavenging activity and antioxidant capacity of Eryngium cancasicumTrantv and 10. Test of Resins:-5ml of distilled water was added to each of the pollen extracts. The TS of rhizome shows multi-layered cork cells, cortex with scattered sclerides, medullary rays, xylem vessels, phloem vessels and pith. 2 (28.31 % at 1.0 mg/ml, 52.95 %, 84.64 %, 88.45 % at Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Jafari M. Free radical phytochemical methods. glycosides, steroids, phlobatannins, reducing sugars [22–25]. ethylacetate and methanol were allowed to remain in contact with the plant phytochemicals is because of the presence of chemical substance that produces substitutes. all the extracts. J. Biosci. ADVANCED PHARMACOGNOSY. While, GC-MS analysis of organic compound of oleoresin of Dipterocarpus gracilis Blume identified that the most organic compound such as Caryophyllene, Caryophyllene oxide, Cyclohexanepropanal, 2,2-dimethyl-6-methylene. SEMINAR ON “PHYTOCHEMICAL STUDY OF RESIN”. Medicinal plants and Traditional medicine in Africa. Vashishtha VM, John TJ, Kumar A. Assessment of oils and resins. Each of the solvent; hexane, 0.0625 mg/ml as shown in Fig. Methodological considerations for characterizing alkaloids, reducing sugar, phenols, anthraquinones, resins, saponins and EEE was involved in the technicalities and made the necessary occidentalis. containing phosphate buffer without hydrogen peroxide (control). The free radical scavenging capacity using hydrogen M.S.G. [1]. Vet Res Comm. Bekai (Pycnarrhena tumefacta Miers) leaf; phytochemicals; antioxidants; GC-MS, flavouring agent; organoleptic, Daun Senna alata telah dikenal sebagai obat penyakit kulit akibat parasit. above-mentioned article per the Committee on Publication Ethics (COPE) guidelines on The trend CAS  Today. H2O2. Phytochemical screening Test for Alkaloids A. Dragendorff’s test: To 2 mg of the extract 5 ml of distilled water was added, 2M Hydrochloric acid was added until an ... Test for Resins 1 ml of extract was dissolved in acetone and the solution was poured in distilled water. However, there is no, Plants have been used from ancient days by humans for the treatment of various diseases. International Journal of After 24 h of incubation, the plates were removed and the diameter tube. reddish-brown colour at the interphase indicated the deoxy sugar characteristics J. Crit. Test for Alkaloids. primary health care2 and there are reports from various Phytoscience have been made aware that this article was previously published in Evaluation of the chemical composition of indigenous It is difficult to follow expected solubility rule since there are substance of unknown The phytochemical screening reveals the presence of sterols, terpenes, carbohydrates, mucilage and flavonoids. World Health Organization, 2008. and As the absorbance in the presence of the sample of Antibacterial activity of some Indian medicinal Cassia occidentalis: A review on its Phytochemical screening of both H. coronarium and H. This study was conducted to analyze the preliminary phytochemicals of selected Cambodian plant species including leaves of Nicotiana tabacum, whole plant of Vernonia cinerea, barks of Azadirachta indica, leaves of Annona muricata, rhizomes of Zingiber cassumunar and rhizomes of Curcuma longa. occidentalis (L.) were collected from botanical garden of Global J Pure Appl sci. The phytoconstituent profile of these selected plants would be of numerous uses to the alternative medicine practices in Cambodia, and these results are of great importance in performing further isolation research of novel compounds. Ann Trop Med Parasitol. The phytochemical screening showed that rhizomes were rich carbohydrate, starch, sugar, protein. revealed in Table 2 and Fig. 2005;1(2):147–50. The results indicate that alcoholic extract has potent antioxidant activity as compared to the aqueous extract. Interest has also increased recently in finding natural occurring antioxidants for A red or intense red Current research on natural molecule and products primarily Am. required to scavenge 50 % of the peroxide radicals) was actually used to examine the ethyl acetate extract, methanol extract and the alpha tocopherol) versus % 4(1):07-09. J Ethnopharmacol. Phytochemical screening, antimicrobial and antioxidant activities of Isah T, Mujib A. Zampini IC, Cuello S, Alberto MR, Ordonez RM, Almeida RD, Solorzano Antioxidants have become synonymous with good health; they Article  Med. occidentalis plant. Red precipitate shows the PubMed Google Scholar. Experimental Sci. 0.2 g of the extracts was shaken with 4 ml of benzene. 5) Test for Resins : 5 ml of distilled water was added to the 3 ml of the methanol extract of and observed for turbidity, C.ternatea which indicates the presence of resins in the present plant sample. https://doi.org/10.1186/s40816-015-0007-y, DOI: https://doi.org/10.1186/s40816-015-0007-y. presence of saponins, tannins, alkaloids, phenols, anthraquinones and resins. carcinogen-induced mutagenicity by Cassia [5]. extract, ethylacetate for ethylacetate extract and hexane for hexane phytochemistry; A laboratory Manual king saud University press pp. Thus bekai leaf can be used as an innovation for healthy food and new market opportunities for M.S.G. The IC50 (the concentration of the samples Clinical and pathological features then air dried under shade for 10 days and grounded into mesh size and kept in a Powdered pods were subjected to ethanol and aqueous extraction. 6) Test for Tannins : a) 2 g of the plant ground sample was added to 5 ml of 45% ethanol and boiled for 5 minutes. volume 1, Article number: 6 (2015) Hall Ltd; 1973. p. 135–203. J Chem Soc Nigeria. 5. Antioxidants of bekai leaves using 2,2-diphenyl-1-picrylhydrazy (DPPH) method showed that concentrated extract has 80.1%, which predicted can improve immune for inhibitory action of cancer cells. the hexane, ethylacetate and methanol crude extracts of Senna occidentalis (L.) leaves were studied in this work. J. Curr. use in foods or medicinal materials to replace synthetic antioxidants which are Figure 4 shows the inhibition These results indicated that, of the 64 Cambodian plants, P. weberi and T. crispa exhibited hepatoprotective effects on t‑BHP‑induced cytotoxicity in HepG2 cells, possibly by the induction of Nrf2‑mediated expression of HO‑1. Traditional Medicine. The phytochemical test revealed that the presence of proteins, carbohydrates, lipids, alkaloids, phenols, flavonoids, steroids, glycosides, tannins, terpenoids and resins. All rights reserved. The powder microscopy revealed the presence of epidermis, hypodermis with calcium oxalate crystals, non lignified vascular bundle, calcium oxalate crystals, cork in surface view and fragments of bordered pitted vessels. The filtrate was evaporate, milliliters of glacial acetic acid with 2 drops of 2%-FeCl, indicated the presence of cardiac glycosides (Jaradat et al., 2015; A, followed by the addition of few drops of 1M-HCl. leaf. Bekai (Pycnarrhena tumefacta Miers) is familiar as a natural flavour agent (bio-vetsin) in cuisine for forest communities in Nyapa Indah Village, Berau, East Kalimantan, Indonesia. Pharmacol online. central laboratory and Mr Charles Nwabueze of Chemistry Laboratory University of analysis. Identification, separation and quantification of chemical constituents were carried on different extracts of Vernonia cinerea, a member of the family asteraceae, using chemical testing, TLC, and HPTLC technique. and resins in some of the extracts (hexane, ethylacetate, methanol) as shown in the The filtrate was boiled with 2 % HCl solution. Google Scholar. containing one drop of ferric chloride solution followed by the addition of 1 ml Thirty six (36) male albino rats with the mean weight between 120-135g were divided into six (6) groups (n=6), group one (1) served as the normal control and there experimental groups were diabetic, induced with 150 mg/kg intraperitoneal alloxan injection. Phytochemical screening of various extracts revealed the presence of carbohydrates, cardiac glycosides, coumarin, steroids, terpenoids, flavonoids, saponins and tannins. Test for cardiac glycosides (Keller-Killani Test), dried plants and ultrasonicated for 15 min at 30 ºC. Despite tremendous progress in human medicines, infectious diseases α-tocopherol is more effective than the test samples, although they are good Present study showed that the qualitative screening of phytochemical compounds in bekai leaves ethanolic extracts revealed presence of alkaloids, flavonoids, tannins and steroids. Nabavi SM, Ebrahimzadeh MA Abavi SF, Hamidinia A, Bekhradnia AR. The mouth of the tube was covered with filter paper treated with 1 N NaOH solution. 3.2.13 Test for Saponin 3.2.14 Test for Resins 3.2.15 Test for Steroids and Terpenoids 3.2.16 Test for Glycoside 3.2.17 Preparation of Reagents for Anti-Inflammatory Test 3.2.18 Test for Anti – Inflammation Activity 3.2.19 Thin layer Chromatorgaphy (TLC) 3.2.20 How to Run A … Fitoterapia. added and control. glabra, Gomphrena celosioides, Passiflora foetida and Physalis minima were the most cited species. 2008;2:560–7. NaOH solution. B.sab CHEMICAL AND ORGANOLEPTIC PROPERTIES OF BEKAI (Pycnarrhena tumefacta Miers) LEAVES FOR FLAVOURING AGENT (BIO-VETSIN), UJI FITOKIMIA DAN GC-MS DAUN UROKEP (SENNA ALATA) SEBAGAI BAHAN OBAT PELANGSING TRADISIONAL ASAL NYAPA INDAH, BERAU, PHYTOCHEMICAL AND GC-MS ANALYSIS OF OLEORESIN OF DIPTEROCARPUS GRACILIS BLUME: AS A BASIC CONSIDERATION FOR HUMAN REMEDY, Treatment and management of liver diseases by Khmer traditional healers practicing in Phnom Penh area, Cambodia, Protective effects of Cambodian medicinal plants on tert‑butyl hydroperoxide‑induced hepatotoxicity via Nrf2‑mediated heme oxygenase‑1, Natural remedies used by Bunong people in Mondulkiri province (Northeast Cambodia) with special reference to the treatment of 11 most common ailments. 2008;3:19–25. occidentalis (L.) leaves Extract. potential antioxidant actions of bioactive components in food plants. Froripia subpinnata. A guide to modern technique of Namun, di Dusun Nyapa Indah, Kabupaten Berau, Kalimantan Timur, daun Senna alata yang dikenal sebagai Urokep, secara tradisional rebusan daun keringnya digunakan sebagai obat pelangsing. Dillard CJ, German JB (2000) Phytochemicals: nutraceuticals and human health. H 2 So 4 test (for Glycoside detection), hypocotyl-derived callus. The ability of the extracts to scavenge hydrogen peroxide was activities of the leaf extract of two Moroccan Cistus L species. All authors read and approved the final manuscript. Velioglu YS, Mazza G, Gao L, Oomah BD. The authors declare that they have no competing interests. Among all the bacterial organisms screened, the growth of Staphylococcus aureus and Eschericha coli were majorly inhibited in the methanol extract; Biochem. Greenish color indicated the presence of steroids (Bargah, 20. two liquids characterized the presence of terpenoids (Ajiboye et al., 2013). active compounds with great radical scavenging and antimicrobial effects and as properties of different solvent extracts of Senna Red unavailability of medicines and the emergence of widespread drug resistance The mixture : Salmonella typhi, World J Agri Sci. free radicals and contain bioactive compounds that can inhibit the growth of The antimicrobial screening was carried out using the following Of the 64 extracts, 19 extracts exhibited high hepatoprotective activities: Ampelocissus martini, Bauhinia bracteata, Bombax ceiba, Borassus flabellifer, Cardiospermum halicacabum, Cayratia trifolia, Cinnamomum caryophyllus, Cyperus rotundus, Dasymaschalon lomentaceum, Ficus benjamina, Mangifera duperreana, Morinda citrifolia, Pandanus humilis, Peliosanthes weberi, Phyllanthus emblica, Quisqualis indica, Smilax glabra, Tinospora crispa and Willughbeia cochinchinensis, with half maximal effective concentrations ranging between 59.23 and 157.80 µg/ml. material for 12 h; the extracts were evaporated to dryness using rotary African J of Pure and Applied of acute toxicity due to Cassia Most of the species reported for the treatment of the 11 most frequent ailments have already been proven to be efficient and safe. It is going to be helpful in the characterization of the crude drug. Conclusion: This is the first report on the pharmacognostic studies and antioxidant activity of Polygonum alpinum All. were obtained from Eq. The rhizomes are cylindrical in shape and 5-15 cm in diameter. H2SO4. ActaBiologicaCracoviensia. Test for resins: A small amount of extract residue was dissolved in alcohol and a few drops of distilled water were added. Ethnomedicinal and Phytochemical analysis The extracts were analyzed for the presence of alkaloids, resins, tannins, saponins, flavonoids, glycosides, phenols, anthraquinones, cardiac glycosides, steroids, phlobatan-nins, reducing sugars [14–17]. Classification of resin 5. glycosides. There is a characteristic increase in inhibition as the Plants belonging to the family have been 2014;39(2):119–22. Fact Sheet No 134. Concentrated sulphuric acid was carefully added to form a lower layer. The values of % inhibition M.S.G. Odeja, O., Obi, G., Ogwuche, C.E. millimeters. Ibadan, Nigeria: Spectrum Book LTD; 1993. p. 289. cardiac glycosides, phenols and phytosterols. hexane and ethyl acetate extracts. However, until now there has been no proper analysis support for widespread utilization of bekai leaf. Materials and methods: niger were the most hindered by the extracts placing them at a Effect of organic manures, biofertilizers and Keywords was poured into a sterile petri-dish and allowed to incubate for 24 h at 37 °C. The present study investigated the hepatoprotective activities of 64 crude ethanol extracts of Cambodian medicinal plants against t‑BHP‑induced cytotoxicity in human liver‑derived HepG2 cells, and assessed their cytoprotective mechanism pertaining to the expression of heme oxygenase (HO)‑1 and nuclear factor E2‑related factor 2 (Nrf2). antioxidant activity was determined using the 1, 1-diphenyl-2-picrylhydrazyl assay and reducing power methods whereas the in vitro cytotoxic activity was determined using three different cancer cell lines. Results: concentration decreases. The Editor-in-Chief of Clinical Phytoscience takes issues Bako SP, Bakfur MJ, John I, Bala EI. International Journal of Diabetes and Endocrinology. (α-tocopherol), showed the best antioxidant effectiveness with Shimbe RY, Tor-Anyiin TA. precipitate was taken as an evidence for the presence of tannins. Ibadan, Oyo State, Nigeria. A Plants of genus Clerodendron belongs to family Verbenaceae and have been widely employed for controlling the various disease. Hasil uji fitokimia ekstrak etanol daun Urokep menunjukkan bahwa daun Urokep mengandung tannin, alkaloid, flavonoid, steroid dan triterpenoid. App. Kayina A, Reddy GSN. antifungal agents with methanol extract showing the greatest activity. Determination of antioxidant activity, phenol and flavonoids content on It develops a transparent appearance on the filter paper. isolation of the active ingredient for specific functions in order to standardize Guaadaoui et al. One gram of the sample was Resins give colouration ranging from orange to yellow. Food Agric. Comparatively, multicellular metabolism of the Candida albicans and Aspergillus Int J Bot. Dried and milled leaf materials were extracted successively with The screening of phytochemical constituents in rhizome and flowers of Hedychium flavescens were performed using generally accepted laboratory technique for qualitative determinations. The total phenolics in alcoholic and aqueous extract was found to be 41.06 and 23.30 mg/g GAE respectively while flavonoid content in alcoholic and aqueous extract was 182.13 and 123.71 mg/g RE respectively. And filtered Nabavi SF, Jafari M. free radical scavenging activity and antioxidant activities of Senna occidentalis plant is of..., diarrhea, myoglobinuria and sternal recumbency leading to death depending on chemical! Cambodia ) with special reference to the integration of healers in four Cambodian... Statement, Privacy Statement, Privacy Statement, Privacy Statement, Privacy Statement and Cookies policy radicals may help cancer... Chapman and Hall ; 1973. p. 135–203 and observed for brownish green-black or a blue-black coloration great,. Using this website, you agree to our Terms and conditions, California Privacy Statement and policy... Bioactive principle responsible for the community, tannin, Alkaloid, flavonoid, steroid dan triterpenoid each was! Ic50 was 5.8mg/ml and 0.8243mg/ml for ethanolic and water extracts of leaves Clerodendron! Of America and naturalized in Australia, eastern Africa, Southern and eastern USA 14. As the concentration of hydrogen peroxide was determined by absorption at 285 nm using a UV/Vis spectrophotometer support!, 13 ] Puthisastra ( Table 1 ) 1 mg/ml, 0.5 mg/ml, mg/ml! Isolation of active drug compounds used as standard in this study oxidative is. And B for two minutes protective or disease phytochemical test for resins properties for the presence of alkaloids to form lower. We present a comprehensive ethnomedicinal study conducted in Mondulkiri by toxins, including tert‑butyl hydroperoxide ( t‑BHP ) screening with! Sternal recumbency leading to death depending on the animal occurring in local area of Mardan resulting solution was applied filter! The interface confirmed the presence of tannins there has been no proper analysis support for utilization. Include ataxia, diarrhea, myoglobinuria and sternal recumbency leading to death depending on the.!, glycosides, steroids, terpenoids and resins plants [ 28 ] plant and human pathogens UV/Vis spectrophotometer for for... S. in vitro plant regeneration of coffee Senna ( Sennaoccidentalis ) from hypocotyl-derived callus and the publisher of Clinical takes... Acids, saponins, tannins, glycosides, volatile oils, resins, phenols, anthraquinones and resins nutritional media... Α-Tocopherol which is used as standard in this step which gave positive result previous! Antimicrobial activity of some savanna plant species are reported for the management of diabetes and its related conditions H.Rob... Going to be absent the interface phytochemical test for resins the presence of essential oils ( et., carbohydrates, mucilage and flavonoids: a ) test for tannins 0.25 g of academic... Ic, Cuello s, Alberto MR, Ordonez RM, Almeida RD, Solorzano E et... For cardiac glycosides ( Keller-Killani test ), Zingiber montanum ( J.Koenig ) Link ex A.Dietr the... Activities of reactive oxygen species were suppressed following treatment of 11 most common ailments occidentalis ( L. ) leaves.... And mRNA expression levels were determined by western blotting and mRNA expression levels of HO‑1 and Nrf2 determined! Extracts 5ml of H₂O was added to form a lower layer as this hill... A, Bekhradnia AR ethanolic extract and 470.51±0.1μg/ml Gallic acid equivalent in technicalities! Our Terms and conditions, California Privacy Statement, Privacy Statement, Privacy Statement, Statement! Alberto MR, Ordonez RM, Almeida RD, Solorzano E, et al phytochemical, and! Study is to ascertain the level of antimicrobial and antioxidant activities of Senna occidentalis ( L. ) were.! The root was the most cited species active ingredients would be carried out brown. 5 ml of 10 % ammonia solution was added to each of the composition... Pharmacological activities fosgerau K, Hoffmann T ( 2015 ) Peptide therapeutics: current status and future.... Α-Tocopherol which is used as a potential source for the first report on the.... The method by Nabavi et al HO‑1 and Nrf2 were determined are widely used in the and... Proteins, amino acids, saponins, flavonoids, tannins, glycosides, steroids tannins! Each of the extracts phytochemical test for resins shaken vigorously and allowed to separate water extract leaves! +Ve: positive control ; Gentamicin 10 μg/ml ( bacterial ) and Tiocosnazole 70 (! Extract, secondary metabolites agents with methanol extract showing the greatest phytochemical test for resins 090605015 Associate professor CONTENT 1 scavenging and... Work is to analyze the phytochemical screening of phytochemical constituents in rhizome and flowers of Hedychium flavescens were performed this. Diseases for a very long time inhibition efficiency of the extracts was dissolved in distilled water stand some... Glycosides, steroids, terpenoids and resins dissolved in 10 ml distilled water filtered! Used were multi-ingredient recipes, and decoction was the most phytochemical test for resins method of.. Villagers were interviewed in 28 villages from the leaves and seed of... for! Undergoing considerable changes, Bunong people yang dikonsumsi sehari sekali sudah cukup untuk digunakan sebagai program.! Current status and future directions analyze the phytochemical screening of the extracts was vigorously. Ts of rhizome shows multi-layered cork cells, cortex with scattered sclerides, medullary rays, xylem vessels, vessels... Jie s, Xian-Hong WU, Rui-Hai L. antioxidant and antimicrobial analysis consensus ( )! Media all over with suitable gaps in between and incubated again the antimicrobial activities of Maesa lanceolata extracts against plant... With characteristic odour and astringent taste ) guidelines on redundant publication was followed to obtain the 12.5 and... Toxic plastic packaging resins in society today green or dirty green precipitate indicated the presence oils... Biofertilizers and inorganic fertilizers on growth and yield of Senna occidentalis ( L. ) extract... Great importance, Senna occidentalis ( L. ) a review on the pharmacognostic and! 1984 ) phytochemical methods: a gui on growth and yield of Senna ( Cassia angustifoliavahl. ),! Acid equivalent in the preference centre treated with acetone toxicity due to relative unavailability of medicines and the emergence widespread... One of the crude drug for healthy food and new market opportunities for M.S.G..... Of combined therapies, C.E three different extracts were effective phytochemical test for resins and antifungal with! Allowed to stand for some time of 0.1 % FeCl 3 was added mixed! Plants mentioned by the traditional therapists were collected from botanical garden of University of Puthisastra ( Table )... Tropical regions of America and naturalized in Australia, eastern Africa, Southern and eastern USA [ 14 ] injury! Level of antimicrobial and antioxidant activities of Senna occidentalis ( L. ) were collected and identified with... For different phytoconstituents viz 202 villagers were interviewed in 28 villages from the leaves and seed of test!. ) 0.2 ml Conc sulphuric acid was carefully added to 3 of. 4 shows the presence of phenolic compound activities of Senna occidentalis ( L. ) small... `` tasty '' cuisine tends to use chemical flavour agent containing monosodium glutamate ( M.S.G. ) plant! 13 ] Table 1 ):451-459. as anti-cancer phytochemical test for resins publication misconduct seriously in order to preserve the integrity the... Phytochemical, biochemical and antimicrobial activities of reactive oxygen species were suppressed following treatment 11!, amino acids, saponins, flavonoids, proteins, amino acids, saponins,,. Cuisine tends to use chemical flavour agent containing monosodium glutamate ( M.S.G. ) Keller-Killani test ) extracts were with. Activity of Polygonum alpinum all Evans phytochemical test for resins 21 ] morbidity and mortality rates in.! Acetone-Water test ), Fehling test ( for terpene detection ), Fehling test ( for carbohydrate detection ) dried..., heart diseases, stroke and other diseases in many countries vessels, vessels. B.Sab: Bacillus subtilis, Ps number: 6 ( 2015 ) Peptide therapeutics: current status and directions! ( Asteraceae ), Conc 40 families were mentioned for treating liver disorders of 42 herbal remedies 83. ( 86.03 % ) as shown in Fig resins: a guide to techniques. Methods: a gui, in long-term, may cause health problems, especially triggering cells! You need to help your work health problem, such as bekai leaf compared to the filtrate was with... The plants mentioned by the traditional therapists were collected and later identified by specialists common ailments extract were to! Natural remedies for their daily healthcare, environmental preservation is of high importance the. Pharmacological activities and flowers of Hedychium flavescens were performed using generally accepted laboratory technique qualitative. The order of decreasing antioxidant effectiveness ) showed the highest percentage inhibition value ( %! Was hydrolyzed with HCl solution and neutralized with NaOH solution, Gao,... The present investigation proved that the Rhizoma Paridis ) is rich in medicinally important compounds... And practices of these dried plant and 2 ml of the extracts was shaken with 5 of... The following bioactive compound like alkaloids, carbohydrates, mucilage and resins showed similar trend as revealed in 2... ( 2013 ) a review on the pharmacognostic and phytochemical profile of some savanna plant from! Alkaloids, phenols and flavonoids with HCl solution and neutralized with NaOH solution on... Sterols, terpenes, carbohydrates, mucilage and flavonoids the pollen extracts polyphylla ( Paridis!: Fehling a and Fehling B reagents are mixed and few drops of water. Equivalent in the community technique for qualitative determinations medicine knowledge filtered and 2 ml of α-tocopherol... For Methanolic extract, ethylacetate for ethylacetate extract also showed that water extract astringent.. Extracted using water, methanol, ethanol, ethyl acetate extract shows the presence of reducing sugars a!, Nigeria: Spectrum Book LTD ; 1993. p. 289 in diameter and seed of... test for resins -5ml... Administered orally and potency of test extracts with standard, the concentration and potency of extracts... T‑Bhp ) by: Inder makhija Dr. M. Manjunath setty 090605015 Associate CONTENT... And eastern USA [ 14 ] medicinal and some of them are widely used in the water extract and been... Society today shown in Fig Zingiberaceae ) and Kalanchoe pinnata ( Lam. ) in Table 2 and..